Toplinska analiza biokompatibilnih materijala za primjenu u tkivnom inženjerstvu

undergraduate thesis

• Title (english): Thermal analysis of biocompatible materials for tissue engineering
• Author: Anamarija Vuković
• Mentor: Tamara Holjevac Grgurić (mentor)
• Committee member: Ivan Brnardić (predsjednik povjerenstva)
• Committee member: Tamara Holjevac Grgurić (član povjerenstva)
• Committee member: Stjepan Kožuh (član povjerenstva)
• Granter: University of Zagreb; Faculty of Metallurgy; Sisak
• Defense date and country: 2019-10-10, Croatia
• Scientific / art field, discipline and subdiscipline: TECHNICAL SCIENCES; Metallurgy

Abstract

Tkivno inženjerstvo pruža velike mogućnosti u razvoju umjetnih organa, osobito bioloških regenerativnih materijala, kao što su hrskavica, kosti i koža. Dizajniranje odgovarajućih biorazgradljivih i biokompatibilnih materijala te praćenje biokemijskih i fizikalnih svojstava, kao i rasta stanica, omogućuje generiranje nadomjestaka koji mogu obnoviti ili znatno poboljšati funkciju oštećenih tkiva.
U ovom radu, istražen je utjecaj različite topografije elektroispredenih polimernih nosača polikaprolaktona (PCL) na uspješnost zasijavanja i rasta stanica. Kolektori su dizajnirani programom 123D Design te su zatim 3D printani. Nosači polikaprolaktona i antibiotika Cefuroxima, CFU®, pripremljeni su elektroispredanjem, u koncentracijama antibiotika, 14 % PCL + 15 % mas. i 14 % PCL + 20 % mas.CFU®. Morfološka struktura pripremljenih nosača prije i nakon zasađivanja HeLa stanica, praćena je skenirajućom elektronskom mikroskopijom (SEM), a biofunkcionalnost nosača ispitana je MTT testom. Temperature kristalizacije (Tk) i taljenja (Tm) PCL/CFU® nosača te stupanj kristalnosti (c) određeni su diferencijalnom pretražnom kalorimetrijom (DSC), dok je toplinska stabilnost materijala praćena tehnikom termogravimetrije (TG). Dobiveni rezultati mikrostrukture nosača korelirani su s rezultatima dobivenih temperatura transformacija te koncentracijom antibiotika, kao i rasprostranjenošću i rastom stanica na nosaču.

Abstract (english)

Tissue engineering provides a great opportunities in the development of artificial organs, especially biological regenerative materials, such as cartilage, bone and skin. Designing the appropriate biodegradable and biocompatible materials and investigation of their biochemical and physical properties as well as cell growth factors, makes it possible to generate substitutes that can restore or substantially improve the function of damaged tissues.
In this paper, the effect of different topographies of electrospun Polycaprolactone fibrous carrier on the incorporation and cell growth was investigated. Polymer carriers with different structure and porosity, have been prepared by electrospinning of PCL/CEFUROXIM® (CFU®) solution at the designed and 3D printed collectors. Polymer solution was prepared with different concentrations of antibiotic CFU®, 15 and 20 %wt. Fibrous morphology was followed by scanning electron microscopy (SEM), while biocompatibility was detected by MTT test. Melting (Tm) and crystallization (Tc) temperatures of PCL/CFU® as well as degree of crystallinity (c) were determined by differential scanning calorimetry (DSC), while thermal stability of material was obtained by thermogravimetry (TG). Results of PCL/CFU® carrier microstructure were correlated with transformation temperatures and antibiotic concentrations as well as cell growth.

• Keywords: tkivno inženjerstvo; elektroispredanje; PCL; Cefuroxim®; biokompatibilnost
• Keywords (english): tissue engineering; electrospinning; PCL; Cefuroxim®; biocompatibility
• Language: croatian
• URN:NBN: urn:nbn:hr:115:752253
• Project: Number: IP-2016-06-6878; Title: Ciljana izrada prototipa vlaknastog nosača za uzgoj tkivnih stanica kombiniranim elektroispredanjem; Acronym: COMBOELECTROSPUN; Leader: Budimir Mijović; Jurisdiction: Croatia; Funder: HRZZ; Funding stream: IP
• Study programme: Title: Metallurgy; specializations in: Metallurgical Engineering, Industrial Ecology; Course: Industrial Ecology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/ prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka metalurgije (sveučilišni/a prvostupnik/ prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka metalurgije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2019-10-14 07:03:41